Ito Ito no Mi provides the user the ability to manipulate people with strings, as well as use the strings as means to cut through matter such as body parts or weapons. The strings can also be used to connect to clouds, thus providing air transportation. The maximum length of these strings is unknown, but is long enough to reach from Green Bit to Dressrosa, as well as connect to the clouds. The strings can be compressed in bundles for various constructs.
The strength of these strings is such that the victims are unable to resist Doflamingo hijacking their mobility, while sharp enough to cut through the flesh and bones of Little Oars Jr. and firearms with ease. Every one of his victims being controlled was unable to resist his powers and therefore was rendered completely helpless. This ability is even powerful enough to stop the physically strong "Diamond" Jozu in his tracks, preventing him from further attacking Crocodile.
As stated by Law, one of this ability's weaknesses is that if there are no clouds in the sky, Doflamingo has nothing to attach his threads to, which in turn prevents him from being able to travel in mid-air. Besides that, the user is still affected by the standard Devil Fruit weaknesses.
Doflamingo usually bends his fingers to move his strings as he pleases for either manipulating people like puppets or to bifurcate his targets. The strings appear to be extremely thin or may even be invisible, making it almost impossible to notice and avoid them. He has shown himself capable of controlling more than one person at the same time without apparent effort.
He can make his victims move to his will by moving his fingers accordingly. Doflamingo mainly uses this power to force comrades to fight (and even kill) each other, while Doflamingo himself sits at the sidelines and watches. It is currently unknown what the limitations of his abilities are.
The strings can also be used for cutting, allowing Doflamingo to effortlessly slice off body parts and destroy weapons. He managed to cleanly sever Little Oars Jr.'s right leg in one swift motion and decapitated Crocodile, though Crocodile's powers only made this a nuisance, due to them not being imbued with Busoshoku Haki; this does not mean Doflamingo cannot, as he brutally injured Smoker by combining his Haki with his strings. When Issho brought down a meteorite on Green Bit, he crossed his strings to form a net, and sliced the meteorite into small pieces to protect himself from harm. He can also compress the strings to create a giant whip or even a perfect clone of himself, capable of fighting alongside him, moving its mouth to appear to be talking, and unraveling for his ultimate technique.
Goshikito (五色糸（ゴシキート）,Goshikīto?, literally meaning "Five Color Strings"): Doflamingo slashes his target with five strings coming out from his fingers. It was first clearly shown being used against the G-5 Marines on Punk Hazard. In the Viz Manga, it is called Penta-Chromatic String.
Tamaito (弾糸（タマイト）,Tamaito?, literally meaning "Bullet String"): Doflamingo points at his opponent with his index finger and fires a string from it, as if he were firing a gun. It was first seen being used in the fight against Law. The attack resembles Shigan, but it has the advantage of a longer range.
Overheat (超過鞭糸（オーバーヒート）,Ōbāhīto?, literally meaning "Excess Whip String"): Doflamingo shoots a thick rope made of strings from his palm and whips it at the target. Overheat's range can go from the sky of Green Bit all the way to Dressrosa, a distance of many kilometers, and is strong enough to cut through a building at that range. It was first seen being used in his fight against Sanji in an attempt to finish him off. The name of this attack is a pun as it ends with "-ito" (string) in the Japanese pronunciation.
Parasite (寄生糸（パラサイト）,Parasaito?, literally meaning "Parasite String"): Doflamingo attaches his strings onto his opponents and manipulates their movements like puppets. This was first seen used to force Vice Admiral Mozambia to attack Vice Admiral Stainless. The strings used are so thin they seem invisible, and are so strong they can completely disable the likes of Jozu and Sanji, even keeping his victims trapped floating in midair, and none of his shown victims were able to resist at all. Furthermore, when used in combination with the BIrd Cage technique, the strings can spread all across the entirety of Dressrosa and control multiple people across the island at the same time without any apparent strain on Doflamingo at all. The technique's name was revealed in the flashback during Doflamingo's takeover of Dressrosa ten years ago, when he demonstrated the ability to take control of King Riku and many of his soldiers at the same time, and from far away. The name of this attack is a pun as it ends with "-ito" (string) in the Japanese pronunciation. This attack was named Marionette (マリオネット,Marionetto?) in One Py Berry Match.
Sora no Michi (空の道,Sora no Michi?, literally meaning "Sky Road"): Doflamingo attaches his strings to clouds, allowing him to move through the air. He was first seen using the technique to travel from Dressrosa to Punk Hazard, but there were not enough clouds for him to be able to complete the journey. When Doflamingo was attacking the Straw Hats and Law at Green Bit, Law instructed the Straw Hats to sail along a route without any clouds, as doing so would prevent Doflamingo from pursuing them.
Marionette (操り人形（マリオネット）,Marionetto?): Doflamingo forms a clone of himself, made entirely out of string. Doflamingo can then manipulate the clone with other strings. The technique was used to survive Kyros' attempted decapitation, and Viola remarked that she had never seen it before herself, suggesting it is rarely used given her ability to see everything going on in the country. Doflamingo has shown great skill in manipulating his clone to orchestrate combined attacks with his real body, such as a double Goshikito.
Torikago (鳥カゴ,Tori Kago?, literally meaning "Birdcage"): Doflamingo's last resort over Dressrosa, in the event Sugar faints and the citizens rebel, which is meant for him to entrap and kill everyone before they could escape and reveal the country's dark secret. From a clone of himself, which he created from a mass bundle of strings, it unravels itself and spreads into the sky. The strings then spread over the outside borders of Dressrosa island, creating a massive razor-sharp wire domed force-field, slicing anyone who tries to pass through it and severs the signals of the Den Den Mushi to the outside world. The strings would then spread into the cage's confines, attaching to the victims within, allowing Doflamingo to use his Parasite technique at a mass scale, forcing them to kill each other.