Pandaman makes a cameo around minute 3:00 (opening included). He is watching Smoker and Ace's confrontation. He also appears around minute 17:00 inside of one Baroque Works's ship running behind some Billions agents.
In this episode, Luffy states that Ace was also in search of One Piece but as the series goes this is not true, where Ace only wanted to become a great and powerful pirate and wanted an answer to know whether if he was meant to be born.
The smileys on Ace's hat are shown both smiling around minute 21:20.
This is the first episode to use Fish as the ending.