As the Straw Hat Pirates sail toward Little Garden, the Baroque Works officer agents Mr. 3 and his partner Miss Goldenweek receive new orders. Finally arriving there, the Straw Hats find the island to be populated by prehistoric animals. Frightened, Nami and Usopp stay behind on the ship as the others spread out to search the island for food and adventure. Nami remembers having read about the island in a book, but by the time she finds it, it is already too late. A giant approaches through the forest.
The Straw Hat Pirates head towards Little Garden. Vivi warns the crew "never to under estimate the sea". Vivi gets mad about how everyone is acting, not taking everything seriously enough. Nami assures her that when things get rough, they know how to handle it. Vivi realizes shes right when a colossal dolphin attacks them, making everyone rush to their stations to outrun the gigantic threat. We then see the mysterious Little Garden island shrouded in fog as everyone gets excited to encounter their second island in their trip on the Grand Line.
We then see Mr. 3 and Miss Goldenweek taking a break on a small resort. They discuss the downfall of Mr 5 and Miss Valentine. He explains to Miss Goldenweek on how Mr. 5 is ignorant, clinging onto his Devil Fruit Powers. He then compliments the people who defeated the duo, stating that they had superior intellect.
Returning to the crew, they remember what Miss All-Sunday said about the island, stating that they will all be dead by the time they reach Alabasta. The crew gets restless as they see more of the island, but keeps on going due to lack of food and materials. As they enter the island, Sanji gets attacked by a gigantic bird signalling that this island isn't normal at all. To reassure that notion, a huge tiger prowls beside the boat and then keels over with blood.
Luffy them demands to visit the island, and Vivi comes with him because she doesn't want to dwell in her problems. Zoro decides to take a walk, as Sanji asks him to get some edible game to bring back. Zoro makes a smart remark and challenges Sanji to see if he can get more game than him. Sanji furious with this embarks onto the island to get more game than Zoro.
Creeped out by the noises, Nami realized that she remembered something about Little Island in a book. Luffy and Vivi find a shellfish like squid, just as giant footsteps are coming from behind, and Sanji nearly gets eaten by a man eating dinosaur.
Vivi then realizes whats happening on the island, saying due to the Grand Line being so dangerous nothing has been able to migrate from the island, preserving the unique eco-system Little Garden has. Zoro and Sanji start their game.
Nami is just about to tell Usopp about what inhabits the island, just as a giant mysterious figure comes through the trees.